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Long PCR Reagents and Guidelines (Modified from Cheng et al. (1)) General Guidelines for Long PCR Conditions and Enzyme Mixtures Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results of Cheng et al. (1) we have had ...
twod.med.harvard.edu/labgc/estep/longPCR_protocol.html
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